CBL208
Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8
clone IT-Ks20.8, Chemicon®, from mouse
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
IT-Ks20.8, monoclonal
Speziesreaktivität
human
Darf nicht reagieren mit
mouse, rat
Hersteller/Markenname
Chemicon®
Methode(n)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotyp
IgG2a
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Angaben zum Gen
human ... KRT20(54474)
mouse ... Krt20(66809)
rat ... Krt20(286912)
Allgemeine Beschreibung
IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Spezifität
The antibody is specific for the 46 kDa polypeptide (cytokeratin 20).
Immunogen
Electrophoretically purified cytokeratin 20 from human intestinal mucosa
Anwendung
Research Category
Zellstruktur
Zellstruktur
Research Sub Category
Cytokeratine
Cytokeratine
Western blotting
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material
Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material
Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.
Optimal working dilutions must be determined by the end user.
Detect Cytokeratin 20 using this Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 validated for use in WB, IH(P).
Verlinkung
Replaces: MABT150
Physikalische Form
Format: Purified
Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.
Lagerung und Haltbarkeit
Once reconstituted, maintain at 2°-8°C for up to 12 months from date of receipt. For extended storage, aliquots can be stored at -20°C. Avoid repeated freeze/thaw cycles.
Hinweis zur Analyse
Control
Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.
Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sie haben nicht das passende Produkt gefunden?
Probieren Sie unser Produkt-Auswahlhilfe. aus.
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
P Harnden et al.
British journal of urology, 78(6), 870-875 (1996-12-01)
To investigate the dysregulation of cytokeratin 20 (CK20) expression in urothelial dysplasia and its potential as a diagnostic aid. Twenty-two patients were selected on the basis that they had undergone one or more biopsies showing dysplasia before the development of
V J Möbus et al.
British journal of cancer, 69(3), 422-428 (1994-03-01)
Two human ovarian (OV-MZ-10, OV-MZ-15) and two colon cancer cell lines (CO-MZ-5, CO-MZ-6) were newly established in permanent cell culture. These cell lines have been maintained in vitro for 5-6 years, the passage number varying from 25 to 228. They
The distribution and origin of keratin 20-containing taste buds in rat and human.
Zhang, C and Oakley, B
Differentiation, 61, 121-127 (1996)
Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins.
Demirkesen, C, et al.
Journal of cutaneous pathology, 22, 518-535 (1995)
I Moll et al.
Archives of dermatological research, 283(5), 300-309 (1991-01-01)
Human eccrine sweat gland ducts and benign and malignant eccrine poromas were studied for the expression of various cytokeratins (CK) and vimentin by applying immunoperoxidase and immunofluorescence microscopy to frozen or paraffin-embedded sections, and using two-dimensional gel electrophoresis and immunoblotting.
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.