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Key Documents

ABT266

Sigma-Aldrich

Anti-Dynein Antibody, HC, cytosolic

from rabbit, purified by affinity chromatography

Synonym(e):

Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

human

Speziesreaktivität (Voraussage durch Homologie)

mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)

Methode(n)

immunocytochemistry: suitable
western blot: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

Allgemeine Beschreibung

Cytoplasmic dynein 1 heavy chain 1 (UniProt Q14204; also known as Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic) is encoded by the DYNC1H1 (also known as CMT2O, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325, SMALED1) gene (Gene ID 1778) in human. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.

Spezifität

This polyclonal antibody targets a sequence within the second AAA domain of cytoplasmic dynein heavy chain encoded by the DYNC1H1 gene.

Immunogen

Epitope: Second AAA domain.
KLH-conjugated linear peptide corresponding to a sequence within the second AAA domain of human cytoplasmic dynein heavy chain.

Anwendung

Research Category
Zellstruktur
Research Sub Category
Cytoskelettale Signalübertragung
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected cytoplasmic dynein heavy chain in HeLa cells.
This Anti-Dynein Antibody, HC, cytosolic is validated for use in Western Blotting, Immunocytochemistry for the detection of Dynein.

Qualität

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected cytoplasmic dynein heavy chain in 10 µg of HeLa cell lysate.

Zielbeschreibung

~500 kDa observed. 532.4 kDa calculated.

Physikalische Form

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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