ABN443

Anti-Glud1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Glutamate dehydrogenase 1, mitochondrial, GDH 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human, mouse
• Speziesreaktivität (Voraussage durch Homologie): monkey (based on 100% sequence homology), bat (based on 100% sequence homology), baboon (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005262
• UniProt-Hinterlegungsnummer: P00367
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GLUD1(2746)

Beschreibung

Allgemeine Beschreibung

GLUD1/GDH1 is a mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha keto-glutarate which is an important intermediate in the TCA cycle. GLUD1/GDH1, because it metabolizes glutamate which is also an important neurotransmitter, plays a role in learning and memory reactions mediated by glutamate, as well as certain neurodegenerative disorders associated with altered glutamate metabolism. Mutations in GLUD1/GDH1 are the most common cause of persistent hyperinsulinism hypoglycemia (HHF6). Interestingly GLUD1/GDH1 may also be involved in glutamate toxicity that accompanies aging. It appears that a decline in GLUD1/GDH1 function, even in over expressing mice, leads to buildup of glutamate and subsequent glutamate induced neurotoxicity.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human Glud1 near the N-terminal.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsabhängige Signalübertragung

Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Glud1 in mouse hindbrain, human thalamus, and human cerebral cortex tissue.

This Anti-Glud1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Glud1.

Qualität

Evaluated by Western Blotting in human hippocampus tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Glud1 in 10 µg of human hippocampus tissue lysate.

Zielbeschreibung

~58 kDa observed

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable