ABN194

Anti-EVI-5 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Ecotropic viral integration site 5 protein homolog, EVI-5
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse, rat
• Speziesreaktivität (Voraussage durch Homologie): human (immunogen homology)
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005656
• UniProt-Hinterlegungsnummer: O60447
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... EVI5(7813)

Beschreibung

Allgemeine Beschreibung

Also known as Ecotropic viral integration site 5 protein homolog, EVI-5 serves as a key regulator of the cell cycle through time-dependent stabilization of Emi1, FBX05 protein, and APC/C ubiquitination substrates, such as cyclin A. EVI-5 accumulates in early G1 following Plk1 destruction, but is degraded in a Plk1- and ubiquitin-dependent manner in early mitosis. EVI-5 ablation causes premature APC/C activation, SCF(betaTrCP)-dependent Emi1 degradation, and cyclin destruction, all of which leads to cell cycle arrest and cytokinesis failure. It exists as a monomer and a dimer, and it may form complexes with alpha- and gamma-tubulin or interact with the INCENP-aurora B kinase-survivin chromosomal passenger complex. EVI-5 is implicated in abnormal states, such as multiple sclerosis and neuroblastoma.

Immunogen

KLH-conjugated linear peptide corresponding to the human EVI-5.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Signalübertragung & Neurowissenschaft

This EVI-5 antibody is validated for use in IHC & WB for the detection of the EVI-5 protein.

Western Blot Analysis: 2 µg/mL from a representative lot detected EVI-5 in NIH/3T3 nuclear extract.

Qualität

Evaluated by Immunohistochemistry in mouse and rat brain tissues.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected EVI-5 in mouse and rat brain tissues.

Zielbeschreibung

~95 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~17 kDa, and ~15 kDa in some cell lysates. This protein is known to have three potential phosphorylation sites.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Mouse and rat brain tissues

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable