ABN1370

Anti-MTNR1B

from rabbit, purified by affinity chromatography

• Synonym(e): Mel-1B-R, Mel1b receptor, MT2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005950
• UniProt-Hinterlegungsnummer: P49286
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MTNR1B(4544)

Beschreibung

Allgemeine Beschreibung

Melatonin receptor type 1B (UniProt: P49286; also known as Mel-1B-R; Mel1b receptor) is encoded by the gene MTNR1B gene (Gene ID 4544) in human. Mel-1B-R is a high affinity receptor for melatonin that belongs to the G-protein coupled receptor 1 family. Mel-1B mediates the reproductive and circadian actions of melatonin. Melatonin is shown to be involved in body mass regulation in mammals and this effect is suggest to be mediated by a direct action of melatonin on Mel-1B receptors on brown adipocytes. Melatonin also acts as a chemopreventive agent and its levels inversely correlate with the risk of developing cancer. In this regard melatonin induces p38-dependent phosphorylation of both p53 and histone H2AX. Activation of the p53-dependent DNA damage response by melatonin is mediated by both Mel-1A and Mel-1B receptors. The absence of either receptor impairs melatonin′s ability to reduce both cell proliferation and clonogenic potential of cancer cells. (Brydon, L. et al. (2001). Endocrinology. 142(10):4264-4271; Santoro, R., et al. (2013). Carcinogenesis. 34(5):1051-1061).

Spezifität

This polyclonal antibody detects melatonin receptor type 1B in human hippocampus membrane.

Immunogen

A KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal end of human melatonin receptor type 1B.

Anwendung

Research Category
Stammzellforschung

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MTNR1B in human retina and human melanoma tissue.

This rabbit polyclonal Anti-MTNR1B, Cat. No. ABN1370 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Melatonin receptor type 1B.

Qualität

Evaluated by Western Blotting in human hippocampus membrane.

Western Blotting Analysis: 2 mg/mL of this antibody detected MTNR1B in 10 mg of human hippocampus membrane.

Zielbeschreibung

~38 kDa observed; 40.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1