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Key Documents

AB7821

Sigma-Aldrich

Anti-Collagen Type VI Antibody

serum, Chemicon®

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

serum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Speziesreaktivität

human

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
immunohistochemistry: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... COL6A1(1291)

Spezifität

Human collagen type I <0.5%

Human collagen type II <0.5%

Human collagen type III <0.5%

Human collagen type IV <0.5%

Human collagen type V 5%

Human collagen type VI 100%

Human fibronectin <0.5%

Human albumin <0.5%

Human immunoglobulin <0.5%

Immunogen

Human placental collagen type VI

Anwendung

Anti-Collagen Type VI Antibody is an antibody against Collagen Type VI for use in ELISA, IH.
Immunohistochemistry: 1:10-1:40 dilution of reconstitute for indirect immunofluorescent staining of frozen tissue sections.

ELISA: 1:10,000 dilution of reconstitute for ELISA on human collagen type VI.

Optimal working dilutions must be determined by the end user.

Physikalische Form

Pooled antisera was cross-absorbed against immobilized human serum proteins and collagen types IV and V. The IgG fraction was then precipitated with ammonium sulfate and subsequently dialyzed. Lyophilized from 500μL of 10mM Na-phosphate, 150mM NaCl, pH 7.5 with 0.1% mannitol.

Lagerung und Haltbarkeit

Maintain lyophilized at -20°C for up to 12 months. Reconstitute with 500uL of distilled water or saline. Store reconstitute between 2 and 8°C for up to two months. Remove any insoluble material by microcentrifugation before use.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Cristina Herrera et al.
PLoS neglected tropical diseases, 10(4), e0004599-e0004599 (2016-04-02)
The time-course of the pathological effects induced by the venom of the snake Bothrops asper in muscle tissue was investigated by a combination of histology, proteomic analysis of exudates collected in the vicinity of damaged muscle, and immunodetection of extracellular
S San Martin et al.
Journal of tissue engineering and regenerative medicine, 7(1), 10-19 (2011-11-05)
Several studies have developed efficient oral mucosa constructs using different types of scaffold. However, the changes in the morphology and gene and protein expression profile that could occur in these artificial constructs remain unknown. This study compared the histology and
Three novel collagen VI chains with high homology to the alpha3 chain.
Gara, SK; Grumati, P; Urciuolo, A; Bonaldo, P; Kobbe, B; Koch, M; Paulsson, M; Wagener, R
The Journal of Biological Chemistry null
Ayman M Ibrahim et al.
Breast cancer research : BCR, 23(1), 90-90 (2021-09-28)
During pregnancy, the mouse mammary ductal epithelium branches and grows into the surrounding stroma, requiring extensive extracellular matrix (ECM) and tissue remodelling. It therefore shows parallels to cancer invasion. We hypothesised that similar molecular mechanisms may be utilised in both
Ai Fujii et al.
Scientific reports, 10(1), 22206-22206 (2020-12-19)
Collagen type VI (COL6) deposition occurs in various glomerular diseases, causing serious pathological damage like nodular lesions. However, the mechanisms underlying the deposition of COL6 remain unclear. In renal biopsy samples, immunohistochemical analyses revealed that COL6 and phosphorylated histone H2AX

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