AB5705

Anti-Calcitonin Gene Related Protein Antibody

Chemicon^®, from chicken

• Synonym(e): CGRP
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: chicken
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunohistochemistry: suitable
• NCBI-Hinterlegungsnummer: NM_001033952.1; NM_001033953.1; NM_001741.2
• UniProt-Hinterlegungsnummer: P01258
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CALCA(796)

Beschreibung

Spezifität

CGRP (Calcitonin Gene Related Peptide).

Immunogen

A 14 amino acid synthetic peptide from rat CGRP I (processed active peptide). The immunogen sequence is identical at 13 of 14 residues between rat/mouse CGRP I and CGRP II. {KDNFVPTNVGSEAF-NH2}

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
ZNS-Kontrolle des Stoffwechsels

Hormone & Rezeptoren

Immunohistochemistry: 1:500 See Protocol below.

Optimal working dilutions must be determined by the end user.



AB5705 has been used successfully on perfusion fixed tissue. Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5705. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

This Anti-Calcitonin Gene Related Protein Antibody is validated for use in IH for the detection of Calcitonin Gene Related Protein.

Physikalische Form

Format: Purified

Purified chicken IgY. Liquid containing 0.05% sodium azide.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1