AB5390

Anti-Sodium Channel Nav1.7 Antibody, Pain

Chemicon^®, from rabbit

• Synonym(e): NENA, NE, SCN9A, PN1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_002977.2
• UniProt-Hinterlegungsnummer: Q15858
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: rat ... Scn9A(78956)

Beschreibung

Spezifität

Recognizes Nav1.7 protein (PN1, NENA, NE, Type IX Voltage Gated Sodium Channel, Scn9a). The epitope is specific for Nav1.7 and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is highly conserved in rabbit and human (14/15 and 13/15 respectively). Other species have not been tested.

Immunogen

Purified peptide from rat Nav1.7 (amino acids 446-460) (Accession AAB50403).

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Ionenkanäle & -transporter

Detect Sodium Channel Nav1.7 using this Anti-Sodium Channel Nav1.7 Antibody, Pain validated for use in IH & WB.

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat dorsal root ganglion (DRG) frozen sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Physikalische Form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Lagerung und Haltbarkeit

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• H-Sätze: H412
• P-Sätze: P273 - P501
• Gefahreneinstufungen: Aquatic Chronic 3
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3