AB3831-I

Anti-phospho Cofilin-1 Antibody (Ser3)

from rabbit, purified by affinity chromatography

• Synonym(e): Cofilin-1, Ser3-phosphorylated, 18 kDa phosphoprotein, Ser3-phosphorylated, Cofilin, non-muscle isoform, Ser3-phosphorylated
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• Methode(n): inhibition assay: suitable (peptide); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005498
• UniProt-Hinterlegungsnummer: P23528
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: phosphorylation (pSer3)
• Angaben zum Gen: human ... CFL1(1072)

Beschreibung

Allgemeine Beschreibung

Cofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.

Spezifität

Target band detection by this polyclonal antibody was prevented by preblocking with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Immunogen

KLH-conjugated linear peptide corresponding to the N-termnal sequence of human cofilin-1 with phosphorylated Ser3.

Anwendung

Research Category
Zellstruktur

Peptide Inhibition Assay: Target band detection in 10 µg of HeLa cell lysate was prevented by preblocking a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

This rabbit polyclonal Anti-phospho Cofilin-1 (Ser3), Cat. No. AB3831-I is validated for use in Peptide Inhibition Assay and Western Blotting for the detection of Cofilin-1 Ser3 phosphorylation.

Qualität

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:200 dilution of this antibody detected Ser3-phosphorylatd cofilin-1 in 10 µg of HeLa cell lysate.

Zielbeschreibung

~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable