AB3226

Anti-Uncoupling Protein 2 Antibody

Chemicon^®, from rabbit

• Synonym(e): UCP2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_003355.2
• UniProt-Hinterlegungsnummer: P55851
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... UCP2(7351)

Beschreibung

Spezifität

Uncoupling Protein 2 (UCP2), human. Human UCP2 is a 309 amino acid mitochondrial uncoupling protein (Fleury et al. 1997; Gimeon et al. 1997). It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). UCP2 may play a critical role in energy balance, body weight, and thermoregulation (Fleury et al. 1997; Gimeon et al. 1997; Flier & Lowell 1997).

SPECIES REACTIVITIES: The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to mouse and rat UCP2 and 92% homology with canine, porcine and bovine.

Immunogen

13 aa peptide sequence near the 3rd and 4th transmembrane domain of human UCP2 (Fleury et al. 1997; Gimeon et al. 1997). The peptide has no significant homology with UCP1, UCP3 or UCP4 (Boss et al. 1997).

Anwendung

Research Category
Stoffwechsel

Research Sub Category
Ionen- & Transportkanäle

Detect Uncoupling Protein 2 using this Anti-Uncoupling Protein 2 Antibody validated for use in ELISA & WB.

Western Blotting: 1-10 μg/mL using Chemiluminescence technique.

Immunohistochemistry: Has not been tested. It is recommended that you use 2-20 μg/mL on formaldehyde fixed tissue.

ELISA: 1:10,000-1:100,000; using 50-100 ng UCP2 control peptide/well.

Optimal working dilutions must be determined by end user.

Physikalische Form

Affinity purified immunoglobulin. Liquid in 100mM Tris, pH 7.5 with 0.2% BSA as stabilizer and 0.05% sodium azide.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable