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Merck

AB16301

Sigma-Aldrich

Anti-Nitric Oxide Synthase III Antibody

Chemicon®, from rabbit

Synonym(e):

NOS III, eNOS

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

human

Hersteller/Markenname

Chemicon®

Methode(n)

immunocytochemistry: suitable
western blot: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... NOS3(4846)

Spezifität

Reactive with human NOS-III (eNOS). This immunogen sequence is 100% homologous in human, bovine, rat, and mouse, but reactivity has not been tested.

Immunogen

Synthetic peptide corresponding to amino acids 596-610 of human endothelial NOS, coupled to KLH.

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Oxidativer Stress
Anti-Nitric Oxide Synthase III Antibody detects level of Nitric Oxide Synthase III & has been published & validated for use in WB & IC.
Western Blot: 5 μg/mL using chemiluminescent detection. Detects ~135-140kDa band in endothelial cell preparations; Higher concentrations of the antibody may be required if colorimetric detection is used. Antibody dilution will also vary with the concentration of the antigen. Immunocytochemistry: Use at 5-10 μg/mL

Optimal working dilutions must be determined by end user.

Physikalische Form

Affinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% Sodium azide as a preservative.

Lagerung und Haltbarkeit

Store at 2-8°C in undiluted aliquots for up to 6 months.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Fahima Syeda et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 166(1), 207-214 (2013-06-04)
The ability to regulate vascular tone is an essential cardiovascular control mechanism, with nitric oxide (NO) assumed to be a ubiquitous smooth muscle relaxant. However, the literature contains reports of vasoconstrictor, vasodilator and no response to nitroergic stimulation in non-mammalian
The interactive effects of hypoxia and nitric oxide on catecholamine secretion in rainbow trout (Oncorhynchus mykiss).
McNeill, B; Perry, SF
The Journal of Experimental Biology null
Morphological support for paracrine inhibition of gastric acid secretion by nitric oxide in humans
Berg, A, et al
Scandinavian Journal of Gastroenterology, 36, 1016-1021 (2001)
Angiotensin II-induced contraction is attenuated by nitric oxide in afferent arterioles from the nonclipped kidney in 2K1C.
Helle, F; Hultstrom, M; Skogstrand, T; Palm, F; Iversen, BM
American Journal of Physiology: Renal Physiology null

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