AB125

Anti-Serotonin Antibody

serum, Chemicon^®

• Synonym(e): 5-HT
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: serum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunohistochemistry: suitable
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Spezifität

Serotonin (5-Hydroxytryptamine)

The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity

5-Hydroxytryptamine-G-PL 1

5-Methoxytryptamine-G-PL 1/25

Tryptamine-G-PL 1/60

5-Hydroxytryptophan-G-PL 1/175

5-Hydroxytryptamine 1/1150

Tryptophan-G-PL 1/3400

5-Methoxytryptophan-G-PL 1/19800



The antisera was also tested for specificity using the free-floating PAP technique on rat

raphe nuclei.



Abbreviations:



(G) Glutaraldehyde

(=) Non-reduced conjugate

(PL) Poly-lysine

Immunogen

5-Hydroxytryptamine-glutaraldehyde-Poly-lysine

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neurotransmitter & Rezeptoren

Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH.

Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.



Optimal working dilutions must be determined by the end user.

Physikalische Form

Rabbit antiserum. Liquid with 0.05% sodium azide.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable