8.55054

EDANS NovaTag^®_resin

Novabiochem^®

• Synonym(e): EDANS-MPB-AM resin

Eigenschaften

• Qualitätsniveau: 200
• Produktlinie: NovaSyn^® TG; Novabiochem^®
• Form: beads
• Eignung der Reaktion: reaction type: Fmoc solid-phase peptide synthesis
• Hersteller/Markenname: Novabiochem^®
• Anwendung(en): peptide synthesis
• Funktionelle Gruppe: amine
• Lagertemp.: 15-25°C

Beschreibung

Allgemeine Beschreibung

The EDANS/dabcyl fluorophore-quencher pair is one of the most commonly-used for FRET peptides, owing to excellent spectral overlap between the emission spectrum of EDANS and absorbance spectrum of dabcyl [1]. The introduction of the EDANS moiety is traditionally achieved either by coupling of a peptide fragment to EDANS in solution ^{[1, 2]} or through the use of a pre-formed derivative, such as Fmoc-Glu(EDANS)-OH ^[3]. Novabiochem′s EDANS resin enables for the first time the direct synthesis of C-terminally EDANS-labeled peptides by solid phase synthesis ^[4]. Loading of the first residue requires acylation of the resin-bound secondary amine and is, therefore, best carried out using HATU activation. Chain extension and cleavage can then be effected using standard methods. EDANS NovaTag resin has been recently employed to prepare fluorescently labeled aminoalkane diphenyl phosphonate affinity probes for chymotrypsin- and elastase-like serine proteases ^[5].

Associated Protocols and Technical Articles
Protocols for Loading of Peptide Synthesis Resins Literature references

^[1] G. T. Wang, et al. (1990) Tetrahedron Lett., 31, 6493.
^[2] C. Garcia-Echeverria & D. H. Rich (1992) Febs Lett., 297, 100.
^[3] J. W. Drijfhout, et al. in ′Peptides, Chemistry, Structure& Biology, Proc. 14th American Peptide Symposium′, P. T. P. Kaumaya &R. S. Hodges (Eds), Kingswinford, Mayflower Scientific Ltd., 1996, pp. 129.
^[4] J. Beythien & P. White (2005) Tetrahedron Lett., 46,101.
^[5] B. F. Gilmore, et al. (2009) Bioconjugate Chem., 20, 2098.

Verlinkung

Replaces: 04-12-3904

Hinweis zur Analyse

Farbe (visuell): weiß bis gelb bis braun
Aussehen der Substanz (visuell): Kügelchen
Beladung (Photometrische Bestimmung des Fmoc-Chromophores nach Abspaltung vom Fmoc-Leu beladenen Harz.): 0.30 - 0.60 mmol/g
Aufquellvolumen (in DMF): chargenspezifischer Wert
Die Polymermatrix ist: Copoly Styrol - 1% DVB 100 - 200 mesh

Rechtliche Hinweise

NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVATAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable