525295

PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)

liquid, clone PY20, Calbiochem^®

• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: PY20, monoclonal
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität (Voraussage durch Homologie): all
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG2b
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: phosphorylation (pTyr)
• Angaben zum Gen: human ... PID1(55022)

Beschreibung

Allgemeine Beschreibung

Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.

Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.

This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

Immunogen

KLH conjugated to phosphotyrosine, glycine, and alanine

Anwendung

ELISA (see comments)

Immunoblotting (1:500-1:1000, see application references)

Frozen Sections (see application references)

Immunocytochemistry (see application references)

Immunoprecipitation (see comments)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 10 mM sodium phosphate, pH 7.4.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
Any cell lysate or tissue extract

Sonstige Hinweise

Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable