444232

MMP-9, Dimer, Human Neutrophil

• Synonym(e): Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase
• EC-Nummer: 3.4.24.35 (BRENDA, IUBMB)
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Assay: ≥90% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: liquid
• Spezifische Aktivität: ≥1000 mU/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Fremdaktivität: other MMP activity, none detected
• Versandbedingung: wet ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.

M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.

Physikalische Form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Angaben zur Herstellung

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Sonstige Hinweise

Olson, M.W. et al. 2000. J. Biol. Chem.275, 2661.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Rechtliche Hinweise

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable