Direkt zum Inhalt
Merck

407654

Sigma-Aldrich

Interleukin-6, Mouse, Recombinant, E. coli

Synonym(e):

rmIL-6

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About This Item

UNSPSC-Code:
12352202

Assay

≥97% (SDS-PAGE)

Qualitätsniveau

Form

lyophilized

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze

Verunreinigungen

≤1 EU/μg Endotoxin (EU/μg IL-6)

Versandbedingung

ambient

Lagertemp.

−70°C

Allgemeine Beschreibung

Recombinant, mouse interleukin-6 expressed in E. coli. Stimulates the production of acute phase proteins by hepatocytes. Induces growth and differentiation of B cells, hepatocytes, keratinocytes, and nerve cells.
Recombinant, mouse interleukin-6 expressed in E. coli. Stimulates the production of acute phase proteins by hepatocytes. Induces growth and differentiation of B cells, hepatocytes, keratinocytes, and nerve cells. Does not inhibit any significant activity on human cells.

Biochem./physiol. Wirkung

ED₅₀ = 20-60 pg/ml as measured in a cell proliferation assay with a factor-dependent plasmacytoma cell line

Warnhinweis

Toxicity: Irritant (B)

Physikalische Form

Lyophilized from sterile-filtered 50 mM NaOAc, 1 mM EDTA, 5% trehalose, 50 µg BSA/µg IL-6 pH 4.0.

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Sonstige Hinweise

Matsuda, T., et al. 1994. Biochem. Biophys. Res. Commun.202, 637.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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T Matsuda et al.
Biochemical and biophysical research communications, 202(1), 637-642 (1994-07-15)
Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein gp130, which associates with the IL-6 receptor alpha chain (IL-6R alpha) in the presence of IL-6. We prepared monoclonal antibodies (mAbs) specific to murine gp130 by immunizing rats and hamsters with

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