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324402

Sigma-Aldrich

Anti-Dopamine D₃ Receptor Rabbit pAb

lyophilized, Calbiochem®

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

serum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

lyophilized

Enthält nicht

preservative

Speziesreaktivität

rat, human

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze

Isotyp

IgG

Versandbedingung

ambient

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... DRD3(1814)
rat ... Drd3(29238)

Allgemeine Beschreibung

Anti-Dopamine D₃ Receptor, rabbit polyclonal, recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D3 dopamine receptor protein.
Recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors.

Immunogen

a synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor

Anwendung

ELISA (see comments)

Flow Cytometry (1:100, see application references)

Immunoblotting (1:500)

Immunohistochemistry (1:5000)

Warnhinweis

Toxicity: Standard Handling (A)

Rekonstituierung

Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Sonstige Hinweise

This antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Jacqueline S Coley et al.
PloS one, 10(2), e0117450-e0117450 (2015-02-04)
Drug abuse is a major comorbidity of HIV infection and cognitive disorders are often more severe in the drug abusing HIV infected population. CD14+CD16+ monocytes, a mature subpopulation of peripheral blood monocytes, are key mediators of HIV neuropathogenesis. Infected CD14+CD16+
Tina M Calderon et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 12(2), 353-370 (2017-01-31)
In human immunodeficiency virus-1 (HIV) infected individuals, substance abuse may accelerate the development and/or increase the severity of HIV associated neurocognitive disorders (HAND). It is proposed that CD14+CD16+ monocytes mediate HIV entry into the central nervous system (CNS) and that

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