264150

Caspase-3 Substrate IV, Fluorogenic

Fluorogenic caspase-3 substrate.

• Synonym(e): Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC
• Empirische Formel (Hill-System): C₃₆H₃₈F₃N₅O₁₄
• CAS-Nummer: 1177935-21-5
• Molekulargewicht: 821.71
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥95% (HPLC)
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Farbe: white to off-white
• Löslichkeit: DMSO: 10 mg/mL
• Fluoreszenz: λ_ex ~400 nm; λ_em ~505 nm
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Biochem./physiol. Wirkung

Cell permeable: no

Primary Target
Fluorogenic caspase-3 substrate.

Product does not compete with ATP.

Reversible: no

Warnhinweis

Toxicity: Standard Handling (A)

Sequenz

Z-Asp-Glu-Val-Asp-AFC

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

Sonstige Hinweise

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable