251760

DAR-4M

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• Synonym(e): DAR-4M, Diaminorhodamine-4M
• Empirische Formel (Hill-System): C₂₅H₂₆N₄O₃
• Molekulargewicht: 430.50
• UNSPSC-Code: 12352202
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥98% (HPLC)
• Form: solution
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: red-violet
• Fluoreszenz: λ_ex ~560 nm; λ_em ~575 nm
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.

Biochem./physiol. Wirkung

Cell permeable: no

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

Warnhinweis

Toxicity: Irritant (B)

Physikalische Form

In DMSO.

Rekonstituierung

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

Sonstige Hinweise

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Rechtliche Hinweise

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): 188.6 °F
• Flammpunkt (°C): 87 °C