234109-M

Colcemid

Cell synchronization agent.

• Synonym(e): Colcemid, Demecolcine
• Empirische Formel (Hill-System): C₂₁H₂₅NO₅
• CAS-Nummer: 477-30-5
• Molekulargewicht: 371.43
• MDL-Nummer: MFCD00075459
• UNSPSC-Code: 12352200

Eigenschaften

• Beschreibung: Merck USA index - 14, 2887
• Assay: ≥98% (HPLC)
• Form: crystalline powder
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Löslichkeit: chloroform: 10 mg/mL; ethanol: soluble
• Lagertemp.: 10-30°C
• SMILES String: N(C1CCc2c(c(c(c(c2)OC)OC)OC)C3=CC=C(C(=O)C=C31)OC)C
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3
• InChIKey: NNJPGOLRFBJNIW-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Biochem./physiol. Wirkung

Primary Target
Depolymerizes microtubules and limits microtubule formation.

Warnhinweis

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.

Sonstige Hinweise

Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214.
Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany