219360

Cathepsin D/E Substrate, Fluorogenic

An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E, but not cathepsins B, H, or L .

• Synonym(e): Cathepsin D/E Substrate, Fluorogenic, MOCAc-GKPILF~FRLK(Dnp)- D-R-NH₂, MOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
• Empirische Formel (Hill-System): C₈₅H₁₂₂N₂₂O₁₉
• Molekulargewicht: 1756.01
• UNSPSC-Code: 12352200

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥97% (HPLC)
• Form: lyophilized solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; desiccated (hygroscopic); protect from light
• Farbe: yellow
• Löslichkeit: DMSO: 5 mg/mL
• Fluoreszenz: λ_ex ~328 nm; λ_em ~393 nm
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

An internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (k_cat/K_m = 15.6 µM^-1s^-1 and 10.9 µM^-1s^-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.

An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (k_cat/K_m = 15.6 µM^-1s^-1 and 10.9 µM^-1s^-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.

Biochem./physiol. Wirkung

Cell permeable: no

Primary Target
Substrate for cathepsins D and E

Product does not compete with ATP.

Reversible: no

kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Standard Handling (A)

Sequenz

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂

MOCAc = (7-Methoxycoumarin-4-yl)acetyl

Physikalische Form

Supplied as a trifluoroacetate salt.

Rekonstituierung

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sonstige Hinweise

Yasuda, Y., et al. 1999. J. Biochem.126, 260.
Yasuda, Y., et al. 1999. J. Biochem.125, 1137.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany