217590

Anti-CD40 Mouse mAb (EA-5)

liquid, ≥95% (SDS-PAGE), clone EA-5

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: EA-5, monoclonal
• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: human
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Isotyp: IgG1
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Anti-CD40, mouse monoclonal, clone EA-5, recognizes ~44 kDa CD40 in Raji cells. It is validated for use in Flow Cytometry, immunoprecipitation, & immunohistochemistry on frozen & paraffin sections.

Protein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.

Recognizes the ~44 kDa CD40 protein in Raji cells.

Immunogen

transfected murine L cells expressing human CD40

Anwendung

Flow cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Paraffin Sections (see comments)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.

Sonstige Hinweise

FACS was performed on 5x10⁵ cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.

Fanslow, W.C., et al. 1992. J. Immunol.149, 655.
Law, C.L., et al. 1990. Leukemia4, 732.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable