207005

Calcineurin Assay Kit, Colorimetric

• Synonym(e): Colorimetric, PP2B Assay Kit
• UNSPSC-Code: 12161503

Eigenschaften

• Verwendung: sufficient for 96 tests
• Qualitätsniveau: 100
• Hersteller/Markenname: Calbiochem^®
• Verpackung: pkg of 96-well plate(s)
• Lagerbedingungen: OK to freeze
• Nachweisverfahren: colorimetric
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

A colorimetric assay kit for measuring calcineurin (PP2B) activity and for inhibitor screening. The assay is performed using RII Phosphopeptide Substrate (Cat. No. 207008).

Verpackung

1 kit in Glass bottle

Komponenten

Human Recombinant Calcineurin, Bovine Brain Calmodulin, RII Phosphopeptide Substrate, Assay Buffer, Phosphate Detection Reagent, Phosphate Calibration Standard, ½ volume 96-Well Plate, and a user protocol.

Warnhinweis

Toxicity: Multiple Toxicity Values, refer to MSDS

Spezifikationen

Assay time: 3.5 h

Lagerung und Haltbarkeit

Store all components except the 96-Well Plate (room temperature) at -70°C for the highest stability. In particular, calcineurin must be handled with utmost care in order to retain its maximal enzymatic activity. Thaw it quickly in a water bath, maintained at room temperature, or by rubbing between fingers, then immediately store on an ice bath. Any remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, we recommend to aliquot the calcineurin into separate tubes and then store at -70°C.

Sonstige Hinweise

Mondragon, A. et al. 1997. Biochemistry 36, 4934.
Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.
Enz, A., et al. 1994. Anal. Biochem. 216, 147.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• H-Sätze: H290
• P-Sätze: P234 - P390
• Gefahreneinstufungen: Met. Corr. 1
• Lagerklassenschlüssel: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 2