189737

Streptavidin, Phycoerythrin Conjugate

This Streptavidin, Phycoerythrin Conjugate is validated for use in Enzyme Immunoassay, Flow Cytometry.

• UNSPSC-Code: 41116133
• NACRES: NA.41

Eigenschaften

• Qualitätsniveau: 100
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: do not freeze; protect from light
• Fluoreszenz: λ_ex 500 nm; λ_em 575 nm
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Streptavidin conjugated to R-phycoerythrin. Specific for biotin, biotinylated proteins, or biotinylated antibodies.

Anwendung

Enzyme Immunoassay (see comments)

Flow Cytometry (1-3 µg per 1 x 10⁶ cells in 100 µl)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 150 mM NaCl, 50 mM sodium phosphate, 1% BSA, 0.1% glycerol, pH 7.6. Ammonium sulfate free.

Rekonstituierung

Do not dilute the entire vial at one time. Dilute only as needed in 50 mM sodium phosphate buffer, pH 7.2, 2-5 mg/ml of BSA or other physiological buffer.

Sonstige Hinweise

Lindmo, T., et al. 1990. J. Immunol. Methods126, 183.
Starling, J.J., et al. 1988. Cancer Res.48, 6211.

The phycobiliprotein used in making this conjugate had a M.W. of 240,000. Molar extinction coefficient at 565 nm: 1.9 x 10⁶. Excitation Max: 500 nm emission max: 575 nm.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable