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Merck

1.52028

Supelco

Chromolith® NH2

L × I.D. 100 mm x 4.6 mm HPLC column

Synonym(e):

Chromolith® Performance NH2 100-4.6 HPLC column

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About This Item

UNSPSC-Code:
41115700

Materialien

PEEK column

Qualitätsniveau

Agentur

suitable for USP L8

Produktlinie

Chromolith®

Leistungsmerkmale

endcapped: no

Verpackung

1 ea of

Kennzeichnungsgrad

5% Carbon loading

Parameter

200 bar pressure
45 °C max. temp.

Methode(n)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

Säule L × ID

10 cm × 4.6 mm

Matrix

monolithic silica

Aktive Matrixgruppe

NH2 bonding phase

Porengröße

13 nm mesoporosity
2 μm macroporosity

operating pH range

2-7.5

Trenntechnik

hydrophilic interaction (HILIC)
normal phase
reversed phase

Lagertemp.

no temp limit

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Allgemeine Beschreibung

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030 with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52030]

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Hinweis zur Analyse

Druck: ≤ 15 bar
Trennleistung (2-Nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretische Bodenzahl (N/m) (Cytosine): ≥ 80000
Kapazitätsfaktor (Cytosine): ≥ 1.3

Rechtliche Hinweise

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

A new HPLC-RI method is proposed for the analysis of Lactose in milk using a Chromolith® NH2 column.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.

Protokolle

Separation of Ribose, United States Pharmacopeia (USP) Reference Standard; Xylitol, analytical standard; Mannose, United States Pharmacopeia (USP) Reference Standard

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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