1.50435

SeQuant^® ZIC^®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 20 mm x 2.1 mm Guard Column

• Synonym(e): Hydrophilic Interaction Liquid Chromatography Guard Column
• UNSPSC-Code: 41115709

Eigenschaften

• product name: SeQuant^® ZIC-HILIC^™ Guard 20 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
• Materialien: PEEK-lined stainless steel column
• Qualitätsniveau: 100
• Agentur: suitable for USP L114
• Produktlinie: SeQuant^®
• Leistungsmerkmale: endcapped: no
• Parameter: 550 bar pressure; 70 °C max. temp.
• Methode(n): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• Säule L × ID: 2 cm × 2.1 mm
• Matrix: fully porous particle
• Aktive Matrixgruppe: ZIC-HILIC bonding phase
• Partikelgröße: 5 μm
• Porengröße: 200 Å pore size
• Betriebs-pH-Wert: 1.5-7.5
• Trenntechnik: hydrophilic interaction (HILIC)
• Lagertemp.: no temp limit

Beschreibung

Allgemeine Beschreibung

The SeQuant^® ZIC^®-HILIC Guard column protects your analytical separation column from contamination.
SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Anwendung

• Development and validation of LC-MS/MS methods to measure tobramycin and lincomycin: Demonstrates the application of SeQuant ZIC-HILIC Guard columns in a pharmacokinetic research study, focusing on the high-precision measurement of pharmaceutical compounds in biological matrices. This method is essential for ensuring accurate dosage and monitoring therapeutic levels, crucial for clinical trials and medical research (Pandey S et al., 2020).

Hinweis zur Analyse

Prüfungsumfang: entspricht

Rechtliche Hinweise

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany