1.25090

Fractogel^® EMD SO₃ ⁻ (M) Harz

RoboColumn Fractogel^® SO3 (M), volume 0.2 mL, prepacked column

• Synonym(e): RoboColumn Fractogel^® SO3 (M), 0.2 ml
• UNSPSC-Code: 41115711

Eigenschaften

• ligand: (Sulfoisobutyl)
• Materialien: polypropylene housing
• Produktlinie: Fractogel^®
• Form: prepacked column
• Parameter: ≤8 bar pressure; ~200 cm/hr max. flow rate
• column I.D. × L: 5 mm × 10 mm
• Volumen: 0.2 mL
• Aktive Matrixgruppe: Methacrylate
• Mittlere Partikelgröße: 40-90 μm
• Kapazität: ~130 mL, resin binding capacity
• Anwendung(en): mAb; recombinant protein; vaccine development
• Trenntechnik: cation exchange
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

5 x 10 mm

Leistungsmerkmale und Vorteile

RoboColumn Fractogel^® SO3 (M), 0.2 ml enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Rechtliche Hinweise

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany