1.10747

GranuCult^® BPLS agar, modified

pkg of 500 g, for Salmonella spp.

• Synonym(e): Brilliant-green Phenol-red Lactose Sucrose Agar, modified
• UNSPSC-Code: 41106213

Eigenschaften

• Form: medium granules (dehydrated (DCM))
• Qualitätsniveau: 200
• Verpackung: pkg of 500 g
• Hersteller/Markenname: GranuCult^®
• Methode(n): microbiological culture: suitable
• pH-Wert: 6.8-7.0 (37 °C, 52 g/L in H₂O, after autoclaving)
• Löslichkeit: 52 g/L
• Schüttdichte: 650 kg/m³
• Anwendung(en): food and beverages; microbiology
• Lagertemp.: 15-25°C
• Eignung: Salmonella spp. (spp.)

Beschreibung

Allgemeine Beschreibung

GranuCult^® BPLS agar (modified) contains Brilliant green that inhibits the growth of the accompanying Gram-positive microbial flora, Salmonella typhi, and Shigella. However, the lower concentration of brilliant green in the modified BPLS agar does not strongly inhibit other Salmonella spp. The medium contains both lactose and sucrose. The color change (red to yellow) in the pH indicator Phenol red indicates the degradation of lactose to acid. Salmonellae do not ferment either lactose or sucrose and appear as red colonies surrounded by a bright red zone. Furthermore, in contrast to BPL agar, the sucrose in the medium allows identification of accompanying, weakly lactose-positive or lactose-negative, but sucrose-positive microorganisms.

Anwendung

GranuCult^® BPLS agar (modified) is a selective agar suitable for the isolation of salmonellae (except for S. typhi) from meat, meat products and other foodstuffs.

Hinweis zur Analyse

Aussehen (Klarheit): klar
Aussehen (Farbe): rot
pH-Wert (25 °C): 6.7 - 7.1
Wiederfindung auf Testmedium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 40 %
Wiederfindung auf Testmedium (Salmonella choleraesuis ATCC 13312): ≥ 40 %
Wiederfindung auf Testmedium (Salmonella enteritidis NCTC 5188 (WDCM 00119)): ≥ 40 %
Wiederfindung auf Testmedium (Escherichia coli ATCC 25922 (WDCM 00013)):
Wiederfindung auf Testmedium (Proteus hauseri ATCC 13315):
Wiederfindung auf Testmedium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 0.01 %
Wiederfindung auf Testmedium (Enterococcus faecalis ATCC 33186): ≤ 0.01 %
Wiederfindung auf Testmedium (Bacillus subtilis ATCC 6633 (WDCM 00003)): ≤ 0.01 %
Kolonienfarbe (Salmonella typhimurium ATCC 14028 (WDCM 00031)): rosa-rot
Kolonienfarbe (Salmonella choleraesuis ATCC 13312): rosa-rot
Kolonienfarbe (Salmonella enteritidis NCTC 5188 (WDCM 00119)): rosa-rot
Kolonienfarbe (Escherichia coli ATCC 25922 (WDCM 00013)): gelb
Kolonienfarbe (Proteus hauseri ATCC 13315): gelb
Bebrütung: 24 Std.; 35 °C; aerob.

Fußnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Rechtliche Hinweise

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3