1.02894

GranuCult^® plus Deoxycholate lactose agar

for coliforms, suitable for microbiology

• Synonym(e): Coliform selective agar
• UNSPSC-Code: 41106213
• NACRES: NA.74

Eigenschaften

• Sterilität: non-sterile
• Qualitätsniveau: 200
• Form: granular
• Verpackung: pkg of 500 g
• Hersteller/Markenname: GranuCult^® plus
• Lagerbedingungen: dry media (Keep tightly closed)
• Methode(n): microbiological culture: suitable
• Farbe: red-brown
• pH-Wert: 6.9-7.3 (25 °C, 57 g/L in H₂O)
• Löslichkeit: 57 g/L
• Anwendung(en): food and beverages; microbiology
• Lagertemp.: 15-25°C
• Eignung: coliforms

Beschreibung

Allgemeine Beschreibung

GranuCult^® plus Deoxycholate lactose agar is a selective agar for the enumeration and isolation of Coliform bacteria from milk, water, ice cream, and other materials. The concentrations of deoxycholate and citrate are so low that the coliform bacteria can grow normally, whereas the accompanying Gram-positive bacteria are inhibited. Degradation of lactose to acid is detected by the pH indicator neutral red, which changes its color to red, and by a zone of precipitation caused by bile acid.

Anwendung

GranuCult^® plus Deoxycholate lactose agar is suitable for the enumeration and isolation of Coliform bacteria from milk, water, ice cream, and other materials.

Leistungsmerkmale und Vorteile

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Hinweis zur Analyse

Aussehen (Klarheit): klar bis schwach opaleszent
Aussehen (Farbe): rotbraun
pH-Wert (25 °C): 6.9 - 7.3
Erstarrungsverhalten (2 Std., 45 °C): flüssig
Wiederfindung auf Testmedium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 40 %
Wiederfindung auf Testmedium (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): ≥ 40 %
Wiederfindung auf Testmedium (Enterobacter cloacae ATCC 13047 (WDCM 00083)): ≥ 40 %
Wiederfindung auf Testmedium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 40 %
Wiederfindung auf Testmedium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≥ 40 %
Wiederfindung auf Testmedium (Shigella flexneri ATCC 12022 (WDCM 00126)): ≥ 40 %
Wiederfindung auf Testmedium (Proteus mirabilis ATCC 14273): ≥ 40 %
Wiederfindung auf Testmedium (Enterococcus faecalis ATCC 11700): ≤ 0.01 %
Wiederfindung auf Testmedium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 0.01 %
Kolonienfarbe (Escherichia coli ATCC 25922 (WDCM 00013)): rot
Kolonienfarbe (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): rosa/rot
Kolonienfarbe (Enterobacter cloacae ATCC 13047 (WDCM 00083)): rosa/rot
Kolonienfarbe (Salmonella typhimurium ATCC 14028 (WDCM 00031)): farblos
Kolonienfarbe (Salmonella enteritidis ATCC 13076 (WDCM 00030)): farblos
Kolonienfarbe (Shigella flexneri ATCC 12022 (WDCM 00126)): farblos
Kolonienfarbe (Proteus mirabilis ATCC 14273): farblos
Präzipitat (Escherichia coli ATCC 25922 (WDCM 00013)): +
Präzipitat (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +/schwach
Präzipitat (Enterobacter cloacae ATCC 13047 (WDCM 00083)): +/schwach
Bebrütung: 24 Std.; 35 °C; aerob.

Sonstige Hinweise

Appearance of Colonies & the corresponding Microorganisms

• Red, surrounded by a zone of precipitate: Lactose-positive: Escherichia coli
• Pale with a pink center and surrounded by a zone of precipitate: Weakly lactose-positive: Enterobacter, Klebsiella, and others
• Colorless: Lactose-negative: Salmonella, Shigella, Proteus, Pseudomonas, and other

Fußnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Rechtliche Hinweise

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2