1.01301

Auramine O (C.I. 41000)

for microscopy

• Synonym(e): 4,4′-Bis-(dimethylamino)-benzophenonimid-hydrochlorid, Pyoktanin
• Empirische Formel (Hill-System): C₁₇H₂₂ClN₃
• CAS-Nummer: 2465-27-2
• Molekulargewicht: 303.83
• MDL-Nummer: MFCD00012484
• UNSPSC-Code: 41116130
• EG-Index-Nummer: 219-567-2

Eigenschaften

• Qualitätsniveau: 400
• Form: solid
• Wirksamkeit: 1000 mg/kg LD₅₀, oral (Rat)
• pH-Wert: 6-7 (20 °C, 10 g/L in H₂O)
• mp (Schmelzpunkt): 265 °C (decomposition)
• Löslichkeit: 10 g/L
• Schüttdichte: 430 kg/m³
• εmax: 1100-1300 at 431-433 nm at 0.005 g/L (pH 6.0)
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-30°C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• InChIKey: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol^™ also has a disinfectant effect, with the result that any microorganisms that are present are killed off. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Anwendung

For microscopy. Staining of mycobacteria according to Hagemann-Hermann, fluorescence staining.
CAS No. 2465-27-2, EC Number 219-567-2.

Hinweis zur Analyse

Identität (UV/VIS-Spektrum)entsprichtAbsorptionsmaximum λ max. (Puffer pH 6.0)431 - 433 nmSpez. Extinktion E1%/1cm (λ_max; 0.005 g/l; Puffer pH 6.0; ber. auf getrocknete Substanz)1100 - 1300DC-PrüfungentsprichtTrocknungsverlust (110 °C)≤ 5 %Eignung für die Mikroskopieentspricht

Rechtliche Hinweise

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS07,GHS08,GHS09
• Signalwort: Warning
• H-Sätze: H302 - H319 - H351 - H411
• P-Sätze: P201 - P202 - P273 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Gefahreneinstufungen: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3