09-310

Anti-phospho-EGFR (Tyr1069) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Receptor tyrosine-protein kinase ErbB-1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor (avian
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): primate (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), pig (based on 100% sequence homology), horse (based on 100% sequence homology)
• Methode(n): ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_005228.3
• UniProt-Hinterlegungsnummer: P00533
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pTyr1069)
• Angaben zum Gen: horse ... Egfr(100067755) ; human ... EGFR(1956) ; mouse ... Egfr(13649) ; pig ... Egfr(397070) ; rat ... Egf(25313)

Beschreibung

Allgemeine Beschreibung

EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Upon ligand binding, EGFR undergoes a transition from an inactive monomeric form to an active dimer. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity that leads to trans- and auto-phosphorylation of tyrosine residues within the cytoplasmic domain of the receptor tyrosine kinase of five tyrosine (Y) residues on sites Y992, Y1045, Y1068, Y1148 and Y1173 located on the C-terminal domain of EGFR. This autophosphorylation elicits downstream activation of several signal transduction cascades, principally the MAPK, Akt and JNK pathways through Ras, PI3 Kinase, and PLC pathways that ultimately leads to the activation of the various pathways downstream. Tyr1069 is phosphorylated during activation.

Spezifität

Reacts strongly and specifically with Tyr1069 phosphorylated EGFR

Immunogen

Epitope: Tyr1069 (cytoplasmic domain)

Tyrosine-phosphorylated epidermal growth factor receptor.

Anwendung

Research Category
Apoptose & Krebs

Zelluläre Signaltransduktion

Research Sub Category
Kinasen & Phosphatasen

Immunofluroescent Immunocytochemistry:

This Anti-phospho-EGFR (Tyr1069) Antibody is validated for use in ELISA, IC, WB for the detection of phospho-EGFR (Tyr1069).

Qualität

Routinely evaluated by Western Blot on untreated and EGF treated A431 lysates.

Western Blot Analysis:
1:1,000 dilution of this antibody detected Tyr1069 phosphorylated EGFR on 10 g of EGF treated A431 lysates.

Zielbeschreibung

~170 kDa

Verlinkung

Replaces: 04-339

Physikalische Form

Antigen Affinity Purified

Antigen affinity purified rabbit polyclonal in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl, with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Hinweis zur Analyse

Control
Untreated and EGF treated A431 lysates

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable