07-520

Anti-eNOS/NOS III Antibody, CT

Upstate^®, from rabbit

• Synonym(e): Constitutive NOS, Endothelial NOS, Endothelial NOS, Endothelial NOS, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: mouse, pig, guinea pig, rat, canine, human, bovine
• Hersteller/Markenname: Upstate^®
• Methode(n): western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NP_000594
• UniProt-Hinterlegungsnummer: P29474
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: bovine ... Nos3(287024) ; dog ... Nos3(403784) ; guinea pig ... Nos3(100135577) ; human ... NOS3(4846) ; mouse ... Nos3(18127) ; pig ... Nos3(397557) ; rat ... Nos3(24600)

Beschreibung

Allgemeine Beschreibung

Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.

Spezifität

Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.

Recognizes eNOS/NOS III, Mr ~135 kDa.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.

Anwendung

Anti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.

Qualität

Routinely evaluated by western blot in RIPA lysates from HUVEC cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.

Zielbeschreibung

135 kDa

Physikalische Form

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Hinweis zur Analyse

Control
Platelets, placenta, liver and kidney.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1