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Key Documents

07-303

Sigma-Aldrich

Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody

Upstate®, from rabbit

Synonym(e):

acetyl-CoA carboxylase 1, acetyl-CoA carboxylase-alpha, acetyl-Coenzyme A carboxylase alpha

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Speziesreaktivität

canine, human

Speziesreaktivität (Voraussage durch Homologie)

bovine (immunogen homology), chicken (immunogen homology), rat (immunogen homology), mouse (immunogen homology), dog (immunogen homology)

Verpackung

antibody small pack of 25 μg

Hersteller/Markenname

Upstate®

Methode(n)

western blot: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

ambient

Posttranslationale Modifikation Target

phosphorylation (pSer79)

Angaben zum Gen

human ... ACACA(31)

Allgemeine Beschreibung

Acetyl CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by the reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones, glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors of the cell, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl CoA carboxylase is thus enhanced. Acetyl CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Spezifität

Recognizes Acetyl CoA Carboxylase phosphorylated at Serine 79, MW 257 kDa.

Immunogen

Epitope: N-terminal
KLH conjugated synthetic peptide (C-HM RSSM[pS]GLHLVK) corresponding to amino acid 73-85 of rat Acetyl CoA Carboxylase.

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
Insulin-/stoffwechselassoziierte Signalübertragung
Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody is an antibody against phospho-Acetyl CoA Carboxylase (Ser79) for use in WB.

Qualität

Evaluated by Western Blot on Lambda phosphatase-treated and untreated A375 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected endogenous phospho-Acetyl CoA Carboxylase (Ser79) in lysate from Lambda phosphatase-treated and untreated A375 cells.

Zielbeschreibung

257 kDa

Verlinkung

Replaces: 04-1009

Physikalische Form

Protein A chromatography
Format: Purified
Protein A purified in buffer containing in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% NaN3.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Rapid transformation of white adipocytes into fat-oxidizing machines.
Orci, Lelio, et al.
Proceedings of the National Academy of Sciences of the USA, 101, 2058-2063 (2004)
Caffeine activates preferentially alpha1-isoform of 5'AMP-activated protein kinase in rat skeletal muscle.
Egawa T, Hamada T, Ma X, Karaike K, Kameda N, Masuda S, Iwanaka N, Hayashi T
Acta Physiologica (Oxford, England) null
Modulation by peroxynitrite of Akt- and AMP-activated kinase-dependent Ser1179 phosphorylation of endothelial nitric oxide synthase
Zou, M. H., et al
The Journal of Biological Chemistry, 277, 32552-32557 (2002)
Therapeutic trial of metformin and bortezomib in a mouse model of tuberous sclerosis complex (TSC).
Auricchio, N; Malinowska, I; Shaw, R; Manning, BD; Kwiatkowski, DJ
Testing null
Caffeine acutely activates 5'adenosine monophosphate-activated protein kinase and increases insulin-independent glucose transport in rat skeletal muscles.
T Egawa, T Hamada, N Kameda, K Karaike, X Ma, S Masuda, N Iwanaka, T Hayashi
Metabolism, Clinical and Experimental null

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