07-2162

Anti-PCNA Antibody

from rabbit

• Synonym(e): proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: rat, mouse, human
• Speziesreaktivität (Voraussage durch Homologie): chimpanzee (based on 100% sequence homology)
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_002583
• UniProt-Hinterlegungsnummer: P12004
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PCNA(5111)

Beschreibung

Allgemeine Beschreibung

Proliferating cell nuclear antigen (PCNA) belongs to the DNA sliding clamp family and is comprised of three identical monomers forming a heterodimer. Due to its unique structure, PCNA is able to increase the processivity of DNA polymerase delta by using its outer domains to be involved in protein-protein interaction while still tethered to and freely sliding along the DNA. PCNA is a critical regulatory component in the onset of leading strand DNA replication and the termination of lagging strand synthesis. Studies have also indicated a role for PCNA in the recruitment and regulation of MMR pathway repair proteins. PCNA is also able to interact with a wide variety of cell cycle proteins and serves to coordinate several proteins participating in DNA processes, such as apoptosis.

Spezifität

Other homologies: Bovine, rabbit, canine, and panda (99% sequence homology).

Immunogen

GST-tagged recombinant protein corresponding to human PCNA.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Use Anti-PCNA Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PCNA also known as proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin.

Western Blot Analysis: 1 µg/mL from a previous lot detected PCNA on 10 µg of NIH/3T3 and PC12 cell lysates.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected PCNA in A431 cells.

Qualität

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PCNA on 10 µg of A431 cell lysate.

Zielbeschreibung

~ 35 kDa observed. PCNA has a predicted molecular weight of 29 kDa, however, it can be monoubiquitinated, resulting in a ~ 35 kDa band.

Physikalische Form

Protein A

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
A431 cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable