07-2129

Anti-phospho-Abi-1 (Ser225) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Abl interactor 1, Abelson interactor 1, Abi-1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Eps8-binding protein, Nap1-binding protein, Nap1BP, Spectrin SH3 domain-binding protein 1, e3B1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• Klon: polyclonal
• application: WB
• Speziesreaktivität: monkey, human
• Methode(n): western blot: suitable

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: monkey, human
• Speziesreaktivität (Voraussage durch Homologie): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001012768
• UniProt-Hinterlegungsnummer: Q8IZP0
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer225)
• Angaben zum Gen: human ... ABI1(10006)

Beschreibung

Allgemeine Beschreibung

Abl interactor 1 (Abelson interactor 1) is a member of the family of Abl-interactors and is highly expressed in the brain. Abl interactor 1 is involved in the signaling of ABL1 and ABL2 nonreceptor tyrosine kinases, and the EGF-receptor. The Abl interactor 1 protein contains SH3 domains, and may function as an adaptor protein; It forms a trimeric complex with EPS8 and SOS1 in the transduction of signals from Ras to Rac. Previous studies have suggested that Abl interactor 1 via the Ras and Rac pathways may regulate cell proliferation, cell adhesion, and actin remodeling. Abl interactor 1 is also investigated for its role in tumor suppression.

Spezifität

This antibody recognizes Abi-1 phosphorylated at Ser225.

Immunogen

Epitope: Phosphorylated Ser225

KLH-conjugated linear peptide corresponding to human Abi-1 phosphorylated at Ser225.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Cytoskelettale Signalübertragung

This phospho-Abi-1 antibody is validated for use in WB for the detection of the phospho-Abi-1 protein.

Western Blot Analysis: A representative lot was used by an independent laboratory in human mammary cells treated with EGF and Abi-1-transfected 293T cells (Mendoza, M., et al. (2011). Molecular Cell 41:661–671).

Qualität

Evaluated by Western Blot in EGF-treated COS cells untreated and treated with lambda phosphatase.

Western Blot Analysis: 1 µg/mL of this antibody detected Abi-1 in 10 µg of EGF-treated COS cells untreated and treated with lambda phosphatase.

Zielbeschreibung

~60 kDa observed.
55 kDa calculated.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
EGF-treated COS cells untreated and treated with lambda phosphatase.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable