06-725-I

Anti-FcεRI α subunit Antibody

serum, from rabbit

• Synonym(e): High affinity immunoglobulin epsilon receptor subunit alpha, Fc-epsilon RI-alpha, FcERI, IgE Fc receptor subunit alpha
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: serum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: human
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001992
• UniProt-Hinterlegungsnummer: P12319
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FCER1A(2205)

Beschreibung

Allgemeine Beschreibung

The allergic reaction is mediated through the IgE high affinity receptor (FcεRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FcεRI receptor complexes causes the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FcεRI receptor is a tetrameric complex, consisting of an a-chain, b-chain and a dimeric g-chain. The a-chain is primarily involved with the binding of IgE, but the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FcεRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

Spezifität

This antibody recognizes the extracellular domain of FcεRI α subunit.

Immunogen

Epitope: Extracellular domain

GST-tagged recombinant protein corresponding to the extracellular domain of human FcεRI α subunit.

Anwendung

Research Category
Entzündung & Immunologie

Research Sub Category
Entzündungs- & Autoimmunmechanismen

Detect FcεRI α subunit using this Anti-FcεRI α subunit Antibody validated for use in Western Blotting, Immunoprecipitation, ICC.

Western Blot Analysis: A 1:5,000 dilution from a representative lot detected FcεRI α subunit in 10 µg of human spleen tissue lysate.

Immunoprecipitation Analysis: A 1:50 dilution from a representative lot immunoprecipitated FcεRI α subunit from human spleen whole lysate.

Flow Cytometry Analysis: A 1:200 dilution from a representative lot detected FcεRI α subunit in RBL cells.

Qualität

Evaluated by Western Blot in RBL cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected FcεRI α subunit in 10 µg of RBL cell lysate.

Zielbeschreibung

~57 kDa observed

Verlinkung

Replaces: 06-725

Physikalische Form

Unpurified

Rabbit polyclonal serum containing 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
RBL cell lysate

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1