05-577
Anti-PP2A Antibody, C subunit, demethylated, clone 4b7
ascites fluid, clone 4b7, Upstate®
Synonym(e):
Anti-NEDLBA, Anti-PP2Ac, Anti-PP2CA, Anti-PP2Calpha, Anti-RP-C
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
ascites fluid
Antikörper-Produkttyp
primary antibodies
Klon
4b7, monoclonal
Speziesreaktivität
vertebrates
Hersteller/Markenname
Upstate®
Methode(n)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... PPP2CA(5515)
Spezifität
unmethylated PP2A, C subunit
Immunogen
peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of human PP2A, C subunit
Anwendung
Research Category
Zelluläre Signaltransduktion
Neurowissenschaft
Zelluläre Signaltransduktion
Neurowissenschaft
Research Sub Category
Kinasen & Phosphatasen
Neurodegenerative Krankheiten
Kinasen & Phosphatasen
Neurodegenerative Krankheiten
Anti-PP2A Antibody, C subunit, demethylated, clone 4b7 is an antibody against PP2A for use in IC, IP & WB.
Qualität
routinely evaluated by immunoblot on RIPA lysates from A431 cells
Zielbeschreibung
36kDa
Physikalische Form
Ascites
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Lagerung und Haltbarkeit
2 years at -20°C
Hinweis zur Analyse
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Rechtliche Hinweise
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sie haben nicht das passende Produkt gefunden?
Probieren Sie unser Produkt-Auswahlhilfe. aus.
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Juyeon Hwang et al.
The Journal of biological chemistry, 291(40), 21008-21019 (2016-08-11)
The protein phosphatase 2A (PP2A) subfamily of phosphatases, PP2A, PP4, and PP6, are multifunctional serine/threonine protein phosphatases involved in many cellular processes. Carboxyl methylation of the PP2A catalytic subunit (PP2Ac) C-terminal leucine is regulated by the opposing activities of leucine
Xiaoyu Xia et al.
Cell cycle (Georgetown, Tex.), 14(12), 1938-1947 (2015-04-04)
Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the
Vitali Stanevich et al.
Molecular cell, 41(3), 331-342 (2011-02-05)
Proper formation of protein phosphatase 2A (PP2A) holoenzymes is essential for the fitness of all eukaryotic cells. Carboxyl methylation of the PP2A catalytic subunit plays a critical role in regulating holoenzyme assembly; methylation is catalyzed by PP2A-specific methyltransferase LCMT-1, an
Ying Zhou et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(12), 16414-16431 (2020-10-19)
Polyphyllin I (PPI) is a natural phytochemical drug isolated from plants which can inhibit the proliferation of cancer cells. One of the PPI tumor-inhibitory effects is through downregulating the expression of Cancerous Inhibitor of PP2A (CIP2A), the latter, is found
X X Yu et al.
Molecular biology of the cell, 12(1), 185-199 (2001-02-13)
Binding of different regulatory subunits and methylation of the catalytic (C) subunit carboxy-terminal leucine 309 are two important mechanisms by which protein phosphatase 2A (PP2A) can be regulated. In this study, both genetic and biochemical approaches were used to investigate
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.