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Merck
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Key Documents

05-384

Sigma-Aldrich

Anti-α-Actinin Antibody, clone AT6/172

clone AT6/172, Upstate®, from mouse

Synonym(e):

Anti-BDPLT15

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

AT6/172, monoclonal

Speziesreaktivität

rabbit, human, mouse

Hersteller/Markenname

Upstate®

Methode(n)

immunocytochemistry: suitable
western blot: suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

NM_001102

UniProt-Hinterlegungsnummer

P12814

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ACTN1(87)

Spezifität

Recognizes α-actinin.

Immunogen

Human α-actinin

Anwendung

Research Category
Zellstruktur
Research Sub Category
Cytoskelett
Immunoprecipitation is not recommended
This Anti-α-Actinin Antibody, clone AT6/172 is validated for use in IC, WB for the detection of α-Actinin.

Qualität

Routinely evaluated in immunoblot on human A431 cells

Zielbeschreibung

100 kDa

Physikalische Form

Protein A purified
Format: Purified
Please see product datasheet for lot-specific formulation.

Lagerung und Haltbarkeit

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy.
Bian, Z; Liao, H; Zhang, Y; Wu, Q; Zhou, H; Yang, Z; Fu, J; Wang, T; Yan, L; Shen, D; Li, H; Tang, Q
Testing null
IKKi deficiency promotes pressure overload-induced cardiac hypertrophy and fibrosis.
Dai, J; Shen, DF; Bian, ZY; Zhou, H; Gan, HW; Zong, J; Deng, W; Yuan, Y; Li, F; Wu et al.
Testing null
Jing Zong et al.
Laboratory investigation; a journal of technical methods and pathology, 93(10), 1128-1136 (2013-08-21)
Nucleotide-binding oligomerization domain-2 (NOD2, also designated CARD15), a member of the NOD-leucine-rich repeat (LRR) protein family (also called the CATERPILLAR family), is upregulated in atheroma lesions and has an important role in regulating the intracellular recognition of bacterial components by
Requirement of phosphatidylinositol 4,5-bisphosphate for alpha-actinin function
Fukami, K, et al
Nature, 359, 150-152 (1992)
Morgane Macheret et al.
Nature, 555(7694), 112-116 (2018-02-22)
Oncogene-induced DNA replication stress contributes critically to the genomic instability that is present in cancer. However, elucidating how oncogenes deregulate DNA replication has been impeded by difficulty in mapping replication initiation sites on the human genome. Here, using a sensitive
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