05-1355
Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10
ascites fluid, clone 3HH4-4C10, from mouse
Synonym(e):
H4KAc, Histone H4 (acetyl), H4 histone family, member N, H4 histone, family 2, histone 2, H4, histone 2, H4a, histone IV, family 2, histone cluster 2, H4a
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
ascites fluid
Antikörper-Produkttyp
primary antibodies
Klon
3HH4-4C10, monoclonal
Speziesreaktivität
human
Speziesreaktivität (Voraussage durch Homologie)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
Methode(n)
immunocytochemistry: suitable
western blot: suitable
Isotyp
IgG1κ
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
acetylation (Lys5/Lys8/Lys12/Lys16)
Angaben zum Gen
human ... H4C1(8359)
mouse ... H4C1(326619)
Allgemeine Beschreibung
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Posttranslational acetylation of the conserved core histone N-terminal tail domains is typically associated with gene activation.
Spezifität
This antibody recognizes Histone H4.
Immunogen
Epitope: Lys5/8/12/16
Linear peptide corresponding to human Histone H4 acetylated at Lys5/8/12/16.
Anwendung
Research Category
Epigenetik & nukleäre Funktionen
Epigenetik & nukleäre Funktionen
Research Sub Category
Histone
Histone
Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.
Use Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10 (mouse monoclonal antibody) validated in WB, ICC to detect acetyl Histone H4 (Lys5/8/12/16) also known as H4KAc, Histone H4 (acetyl).
Qualität
Evaluated by Western Blot in HeLa acid extract lysate.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.
Zielbeschreibung
~ 13 kDa
Physikalische Form
Unpurified
Unpurified mouse monoclonal IgG1κ preservative-free ascites.
Lagerung und Haltbarkeit
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Hinweis zur Analyse
Control
HeLa acid extract lysate
HeLa acid extract lysate
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Scientific reports, 7(1), 9581-9581 (2017-08-31)
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iScience, 21, 773-788 (2019-11-16)
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