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04-135

Sigma-Aldrich

Anti-Brachyury Antibody, clone 3E4.2

clone 3E4.2, Upstate®, from mouse

Synonym(e):

T brachyury (mouse) homolog, T brachyury-like, T protein, T, brachyury homolog (mouse), transcription factor T

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

3E4.2, monoclonal

Speziesreaktivität

human

Hersteller/Markenname

Upstate®

Methode(n)

western blot: suitable

Aufnahme

sample type mesenchymal stem cell(s)

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... TBXT(6862)

Allgemeine Beschreibung

The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Spezifität

Reactivity with other species has not been tested.
This monoclonal antibody recognizes the brachyury protein.

Immunogen

Epitope: C-terminus
Synthetic human brachyury peptide, a.a. 224-435.

Anwendung

Research Category
Stammzellforschung
Research Sub Category
Mesenchymale Stammzellen
This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.

Qualität

Routinely evaluated by Western blot on NT2 cell lysates.

Western Blotting:
Recommended working dilutions are 1:1000.

Zielbeschreibung

47 kDa

Physikalische Form

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Lagerung und Haltbarkeit

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Brachyury, a crucial regulator of notochordal development, is a novel biomarker for chordomas.
Vujovic, S, et al.
The Journal of Pathology, 209, 157-165 (2006)
Chromatin remodeling during mouse and human embryonic stem cell differentiation.
Golob, Jonathan L, et al.
Developmental Dynamics, 237, 1389-1398 (2008)
T P Yamaguchi et al.
Genes & development, 13(24), 3185-3190 (2000-01-05)
Wnt3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is required for paraxial mesoderm development. In its absence cells adopt ectopic neural fates. Embryos lacking the T-box-containing transcription factors, Brachyury or Tbx6
Development of antibodies to human embryonic stem cell antigens.
Cai, Jingli, et al.
BMC Developmental Biology, 5, 26-26 (2005)
Hematopoiesis from human embryonic stem cells: overcoming the immune barrier in stem cell therapies.
Priddle, Helen, et al.
Stem Cells, 24, 815-824 (2006)

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