906603
DLAM-Iδ1Tγ-13CH3 Methyl Labeling Kit
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About This Item
UNSPSC-Code:
12352200
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Allgemeine Beschreibung
DLAM-Iδ1Tg-13CH3 kit has 13CH3isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.
Anwendung
DLAM-Iδ1Tg-13CH3 is an optimized kit to produce proteins with 13CH3 isotopomer on the methyl groups of isoleucine and threonine. This kit has been tested with protein isotopic labeling in E. coli. It is used in methyl-TROSY NMR for the investigation of the active site of the proteasome and also in the study of catalytic mechanism.
For detection of long-range nOes or for the NMR study of large proteins
Verpackung
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
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Algirdas Velyvis et al.
Journal of the American Chemical Society, 135(25), 9259-9262 (2013-06-27)
The 20S proteasome core particle is a molecular machine that plays a central role in the regulation of cellular function through proteolysis, and it has emerged as a valuable drug target for certain classes of cancers. Central to the development
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that
Artikel
Methyl Isotope-Labeling of Proteins from NMR-Bio
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
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