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HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18 application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

53829-U

Colonne de CLHP Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Étalon

Réf. du produit
Description
Tarif

F7876

Folic acid

≥97%

M9929

Méthotrexate hydrate

meets USP testing specifications

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

5 μL

Description

Remarque sur l'analyse

This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

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