- Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.
Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.
Bioorganic & medicinal chemistry letters (2000-12-29)
J E Sheppeck, H Kar, L Gosink, J B Wheatley, E Gjerstad, S M Loftus, A R Zubiria, J W Janc
PMID11128641
RÉSUMÉ
A statistically exhaustive, 8800 compound tripeptidal amidomethylcoumarin library was synthesized as discreet compounds using solid-phase combinatorial chemistry. A subset of the compounds was purified by HPLC and tested in a high-throughput fluorometric assay against several known serine and cysteine proteases to demonstrate the utility of this library for profiling protease substrate specificity.
MATÉRIAUX
Référence du produit
Marque
Description du produit
Sigma-Aldrich
L-Citrulline 7-amido-4-methylcoumarin hydrobromide, ≥98.0% (sum of enantiomers, HPLC)