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TOYOPEARL® HW-40C Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 75 μm particle size

Synonyme(s) :

TOYOPEARL® HW-40 Size Exclusion Media

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About This Item

Code UNSPSC :
41115700

Description

HW-40C

Gamme de produits

TOYOPEARL®

Forme

slurry

Conditionnement

bottle of 500 mL

Paramètres

3 bar max. pressure

Technique(s)

HPLC: suitable

Matrice

hydroxylated methacrylic polymer

Groupe de la matrice active

hydroxylated methacrylate phase

Taille des particules

75 μm

Dimension de pores

100-7000 Da MW range

pH de fonctionnement

2-13

Technique de séparation

size exclusion (SEC)

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Description générale

Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

Application

Toyopearl media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

Caractéristiques

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Forme physique

Shipped in 20% (v/v) ethanol.

Produits recommandés

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

Toyopearl is a registered trademark of Tosoh Corporation

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

104.0 °F - closed cup

Point d'éclair (°C)

40 °C - closed cup

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
117H0814

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Sigma-Aldrich

G10120

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M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was

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