ZRB2132

Anti-Ago 1 Antibody, clone 1L3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Protein argonaute-1;Argonaute1;hAgo1;Argonaute RISC catalytic component 1;Eukaryotic translation initiation factor 2C 1;eIF-2C1;eIF2C1;Putative RNA-binding protein Q99
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1L3, recombinant monoclonal
• Description: 1L3 Clone
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 97.21 kDa; observed mol wt ~105 kDa
• Produit purifié par: using Protein A
• Espèces réactives: monkey, human, mouse
• Réactivité de l'espèce (prédite par homologie): bovine, sheep, feline
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: Internal
• Numéro d'accès Protein ID: NP_001304051
• Numéro d'accès UniProt: Q9UL18
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AGO1(26523)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1L3 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Protein argonaute-1 (Ago 1). It targets an epitope within 21 amino acids from the internal region.

Immunogène

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Protein argonaute-1 (Ago 1).

Application

Quality Control Testing

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in K562 and COS-7 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in human placenta tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound Ago1 peptide with a KD of 3.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.

Description de la cible

Protein argonaute-1 (UniProt: Q9UL18; also known as Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding protein Q99) I encoded by the AGO1 (also known as EIF2C1) gene (Gene ID: 26523) in human. The highly conserved Argonaute proteins play a critical role in the regulation of gene expression and orchestrate the establishment and the maintenance of cell identity throughout the entire life cycle. In humans, four functional Argonaute proteins (Ago 1-4) have been described that exhibit high structure similarity, but also have a few non-conserved amino acids in their functional domains. In contrast to Ago 2, which has two N-terminal motifs that are required for its full catalytic activity, Ago1 contains only one N-terminal motif and Ago 3 and 4 lack these motifs and are catalytically inactive. About 80% of Ago1 clusters are associated with cell-type-specific transcriptional enhancers. Ago1 is required for RNA-mediated gene silencing. It binds to short RNAs such as miRNA or siRNA and represses the translation of mRNAs which are complementary to them. Its PIWI domain is localized in amino acids 515 to 816. Ago1 lacks RNA cleavage activity due to the absence of the conserved histidine at position 805. Ago1 is shown to be enriched around transcription start sites and binds preferentially to active transcriptional enhancers. Its association with enhancers is mediated by RNAs that are transcribed from these enhancers. However, the interaction of Ago1 with enhancers does not appear to regulate transcription of the neighboring genes. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Müller, M., et al. (2020). Front. Cell Dev. Biol. 7; 360; Allo, M., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(44); 15622-15629).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable