ZRB1968

Anti-MRP1 Antibody, clone 1H8 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Multidrug resistance-associated protein 1;EC:7.6.2.2;ATP-binding cassette sub-family C member 1;Glutathione-S-conjugate-translocating ATPase; ABCC1;Leukotriene C(4) transporter;LTC4 transporter
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1H8, recombinant monoclonal
• Description: 1H8 Clone
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 171.59 kDa; observed mol wt ~200 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_004987
• Numéro d'accès UniProt: P33527
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MRP1(4363)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1H8 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Multidrug resistance-associated protein 1 (MRP1). It targets at epitope within 22 amino acids from the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Multi-drug resistance-associated protein 1 (MRP1).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MRP1 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MRP1 in Panc-1 cell lysate.

Flow Cytometry Analysis: 1 μg from a representative lot detected MRP1 in one million Hela cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRP1 in A431 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MRP1 in HeLa cell lysate.

Description de la cible

Multidrug resistance-associated protein 1 (UniProt; P33527; also known as EC:7.6.2.2, ATP-binding cassette sub-family C member 1, Glutathione-S-conjugate-translocating ATPase ABCC1, Leukotriene C(4) transporter, LTC4 transporter) is encoded by the MRP1 (also known as ABCC1, MRF) gene (Gene ID: 4363) in human. MRP1 is a multi-pass membrane glycoprotein of ABC transporter superfamily that contains nine extracellular domains, seventeen transmembrane domains, and nine cytoplasmic domains. It mediates the export of organic a It is ubiquitously expressed in almost all human tissues. Anions and drugs from the cytoplasm and confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. It is also reported to catalyze the export of sphingosine 1-phosphate from mast cells independently of their degranulation. MRP1 also mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-β-o-glucuronide, and antiviral drugs. Elevated levels of MRP1 and/or mRNA levels have been reported in many hematologic and solid tumors, and in some instances, and are predictors of poor response to chemotherapy. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cole, SPC. (2014). J. Biol. Chem. 289(45); 30880-30888; Mitra, P., et al. (2006). Proc. Natl. Acad. Sci. USA. 103(44); :16394-16399).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable