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ZRB1690

Sigma-Aldrich

Anti-SRRM4 Antibody, clone 1I2 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

Serine/arginine repetitive matrix protein 4;Medulloblastoma antigen MU-MB-2.76;Neural-specific serine/arginine repetitive splicing factor of 100 kDa;Neural-specific SR-related protein of 100 kDa; nSR100

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

1I2, recombinant monoclonal

Description

recombinant, expressed in HEK 293 cells

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 68.56 kDa
observed mol wt ~65 kDa

Produit purifié par

using Protein A

Espèces réactives

human, rat, mouse

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG

Séquence de l'épitope

N-terminal

Numéro d'accès Protein ID

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SRRM4(84530)

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1I2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Serine/arginine repetitive matrix protein 4 (nSR100/SRRM4). It targets an epitope within 18 amino acids from the N-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Serine/arginine repetitive matrix protein 4 (nSR100/SRRM4).

Application

Quality Control Testing

Evaluated by Western Blotting in Rat Kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SRRM4 in Rat Kidney tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SRRM4 in Human Liver, Mouse Liver, and Human Kidney tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SRRM4 in A431, HeLa, and HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SRRM4 in human kidney tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound SRRM4 peptide with a KD of 1.2 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in Rat Kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SRRM4 in Rat Kidney tissue lysate.

Description de la cible

Serine/arginine repetitive matrix protein 4 (UniProt: A7MD48; also known as Medulloblastoma antigen MU-MB-2.76, Neural-specific serine/arginine repetitive splicing factor of 100 kDa, Neural-specific SR-related protein of 100 kDa, nSR100) is encoded by the SRRM4 (also known as KIAA1853) gene (Gene ID: 84530) in human. SRRM4 is a nuclear protein that is specifically expressed in neuronal cells. It plays an important role during embryonic development and in the proper functioning of the adult nervous system. It serves as a splicing factor that is specifically required for neural cell differentiation. It acts in conjunction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promotes neural-specific exon inclusion in many genes that function in neural cell differentiation. It is shown to regulate the splicing of protrudin gene (Zfyve27) transcripts in neuronal cells and promotes splicing of protrudin pre-mRNA so as to include a microexon (exon L) encoding seven amino acids in a neuron-specific manner. The resulting protein known as Protrudin-L promotes neurite outgrowth during neurogenesis. Its depletion in Neuro2A cells is shown to impair neurite outgrowth, whereas its ablation in mouse embryos is reported to prevent differentiation of neuronal progenitors in the cortex and impairs the development of the central and peripheral nervous systems. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ohnishi, T et al. (2017). Sci. Rep. 7: 41130; Quesnel-Vallières, M et al. (2015). Genes Dev. 29(7); 746-759).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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