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ZRB1636

Sigma-Aldrich

Anti-Cathepsin L1 Antibody, clone 1G2 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

EC:3.4.22.15;Major excreted protein;MEP

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

1G2, recombinant monoclonal

Description

recombinant, expressed in HEK 293 cells

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 37.56 kDa
observed mol wt ~25, 32, 37 kDa

Produit purifié par

using Protein A

Espèces réactives

human, rat

Réactivité de l'espèce (prédite par homologie)

monkey

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotype

IgG

Séquence de l'épitope

C-terminal half

Numéro d'accès Protein ID

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1G2 is a Rabbit recombinant monoclonal antibody that specifically detects Cathepsin L. It targets an epitope within 14 amino acids from the C-terminal half. It detects procathepsin L, mature Cathepsin L, and Cathepsin L heavy chain.

Immunogène

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human Cathepsin L.

Application

Quality Control Testing

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cathepsin L1 in PC12 cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cathepsin L1 in A549 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound Cathepsin L1 peptide with a KD of 4.1 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cathepsin L1 in A549 cells

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cathepsin L1 in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

Procathepsin L (UniProt: P07711; also known as EC:3.4.22.15, Cathepsin L, Major excreted protein, MEP) is encoded by the CTSL (also known as CTSL1) gene (Gene ID: 1514) in human. Cathepsin L is a lysosomal protease that is synthesized as a procathepsin with a signal peptide (aa 1-17) and two propeptides (aa 18-113 activation peptide; and 289-291) that are cleaved off to produce mature active enzyme. After the removal of these peptides, cathepsin can exist in three forms: cathepsin L (aa 114-333), cathepsin L heavy chain (aa 114-288) and cathepsin L light chain (aa 292-333). The heavy and light chains are connected by disulfide bonds. Active cathepsin L is a thiol protease with endopeptidase activity and preferentially cleaves peptide bonds with aromatic residues in P2 position and hydrophobic residues in P3 position. It is catalytically active in the pH range 3 to 6.5. It participates in overall degradation of proteins in lysosomes and plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. It is also Involved in the solubilization of cross-linked thyroglobulin and in subsequent release of thyroxine (T4) by limited proteolysis of thyroglobulin in the thyroid follicle lumen. Cathepsin L has been implicated in several pathological conditions, including invasion and tumor metastasis, atherosclerosis, inflammation, and degradation of extracellular matrix that is reported to be an important process in entry of SARS-CoV-2 into host cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gomes, CP., et al. (2020). Front. Cell. Infect. Microbiol. 10; 589505; Turk, V., et. al. (2012). Biochim. Biophys. Acta. 1824(1); 68-88).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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