ZRB1338

Anti-MCU Antibody, clone 3N3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Calcium uniporter protein mitochondrial;HsMCU;Coiled-coil domain-containing protein 109A
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 3N3, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 39.87 kDa; observed mol wt ~41 kDa
• Produit purifié par: using Protein A
• Espèces réactives: mouse, human
• Réactivité de l'espèce (prédite par homologie): bovine, canine, rat, feline, monkey
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_612366
• Numéro d'accès UniProt: Q8NE86
• Autre catégorie plus écologique: , Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 3N3 is a Rabbit recombinant monoclonal antibody that specifically detects Calcium uniporter protein, mitochondrial (MCU). It targets an epitope within 17 amino acids from the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Calcium uniporter protein, mitochondrial (MCU).

Application

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MCU in A549 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MCU in PANC-1 and NIH3T3 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound MCU with a KD of 2.0 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MCU in A549 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

Calcium uniporter protein, mitochondrial (UniProt: Q8NE86; also known as HsMCU, Coiled-coil domain-containing protein 109A, MCU) is encoded by the MCU (also known as C10orf42, CCDC109A) gene (Gene ID: 90550) in human. MCU is a multi-pass inner mitochondrial membrane calcium uniporter that mediates calcium uptake into mitochondria. It is synthesized with a transit peptide (aa 1-50), which is subsequently cleaved off to generate the mature MCU protein that has two mitochondrial matrix domains (aa 51-233 and 284-351), two transmembrane domains (aa 234-256 and 266-283) and one mitochondrial Intermembrane domain (aa 257-265). Its activity is regulated by the mitochondrial calcium uptake 1 (MICU1) and MICU2. MICU1 is a single pass membrane protein that helps to stabilize the entire mitochondrial calcium uniporter complex. It also limits the amount of calcium that enters the cell during low concentrations of calcium. Also, it functions alongside MCU to keep the accumulated calcium inside the mitochondrial matrix. MICU2 works with MICU1 and MCU to reduce the amount of calcium coming into the matrix. At low calcium levels MCU activity is down-regulated by MICU1 and MICU2 and at higher calcium levels MICU1 increases MCU activity. Synaptic activity is also reported to transcriptionally represses MCU gene, via a mechanism involving the nuclear Ca2+ and CaM kinase-mediated induction of Npas4, resulting in the inhibition of NMDA receptor-induced mitochondrial Ca2+ uptake and preventing excitotoxic death. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Marchi, S., and Pinton, P. (2014). J. Physiol. 592(5); 829-839; Qiu, J., et al. (2013). Nat. commun. 4; Article 2034; De Stefani, D., et al. (2011). Nature 476 (7360); 336-340).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable