U7258

Anti-Ubiquilin antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-DA41, Anti-DSK2, Anti-Protein-linking IAP to cytoskeleton 1, PLIC-1, Anti-UBQLN1, Anti-XDRP1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~62 kDa
• Espèces réactives: human
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Concentration: ~0.5 mg/mL
• Technique(s): western blot: 0.5-1.0 μg/mL using lysates of HEK-293T cells and COS7 cells expressing human ubiquilin-1.
• Numéro d'accès UniProt: Q9UMX0
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... UBQLN1(29979)

Description

Description générale

Ubiquilin-1 (UBQLN1) is a ubiquitin-like (UBL) protein, that contains UBL and ubiquitin-associated (UBA) domains in its N- and C-termini, respectively. UBQLN1 gene is located on the human chromosome at 9q21.32.

Spécificité

Anti-Ubiquilin-1 specifically recognizes human ubiquilin-1.

Application

Anti-Ubiquilin-1 antibody produced in rabbit has been used in:

• immunoblotting
• immunohistochemistry
• immunofluorescence

Actions biochimiques/physiologiques

Ubiquilin-1 (UBQLN1) protein plays a major role in regulating the proteasomal degradation of several proteins including the presenilins PS1 and PS2. This protein is also involved in the degradation of cyclin A, γ-aminobutyric acid (GABA) receptor, and hepatitis C virus RNA-dependent RNA polymerase proteins. This protein might play a general role in neurodegenerative diseases. UBQLN1 genetic variants are associated with an increased risk of Alzheimer′s disease (AD). Downregulation of UBQLN1 gene modulates PS1 endoproteolysis. Downregulation of this gene is also involved in regulating the protein levels of nicastrin and presenilin enhancer 2 (PEN-2). It also increases the rate of β-amyloid precursor proteins (APP) maturation and trafficking through the secretory pathway associated with increased production of secretory β-amyloid precursor proteins (sAPP) and amyloid β (Aβ). UBQLN1 protein is associated with neurofibrillary tangles in AD brain and Lewy bodies in Parkinson′s disease (PD).

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves